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KMID : 0545120140240010048
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 1 p.48 ~ p.51
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Purification and Characterization of α-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007
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Osamu Ariga
Naoki Okamoto
Naomi Harimoto
Kiyohiko Nakasaki
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Abstract
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α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32ºC and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35ºC for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
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KEYWORD
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Cellvibrio sp., α-NAOS hydrolase, neoagarooligosaccharide
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